Precision Cut Lung Slices as a Model for 3R Application in Toxicology
نویسندگان
چکیده
منابع مشابه
Biaxial distension of precision-cut lung slices.
The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
متن کاملElectric field stimulation of precision-cut lung slices.
The precision-cut lung slice (PCLS) technique is widely used to examine airway responses in different species. We developed a method to study nerve-dependent bronchoconstriction by the application of electric field stimulation (EFS) to PCLS. PCLS prepared from Wistar rats were placed between two platinum electrodes to apply serial rectangular impulses (5-100 Hz), and bronchoconstriction was stu...
متن کاملReproducible uniform equibiaxial stretch of precision-cut lung slices.
Simulating ventilator-induced lung injury (VILI) in the laboratory requires stretching of lung alveolar tissue. Whereas precision-cut lung slices (PCLSs) are widely used for studying paracrine signaling pathways in the lungs, their use in stretch studies is very limited because of the technical challenge of fixing them to a stretchable substrate, stretching them uniformly, or holding them in a ...
متن کاملIL-13 induced asthma model in human precision-cut lung slices
O. Danov1, S. M. Jiménez Delgado1, H. Drake1, S. Seehase2, A. M. Hohlbaum3, C. Bersch4, D. Jonigk5, G. Warnecke6, A. Braun1 and K. Sewald1 1Airway Immunology, Fraunhofer Institute for toxicology and experimental Medicine, Biomedical Research in endstage and Obstructive lung Disease Hannover (BReAtH), Member of the German Centre for lung Research (DZl), Hannover, Germany; 2Clinical & experimenta...
متن کاملan application of equilibrium model for crude oil tanker ships insurance futures in iran
با توجه به تحریم های بین المملی علیه صنعت بیمه ایران امکان استفاده از بازارهای بین المملی بیمه ای برای نفتکش های ایرانی وجود ندارد. از طرفی از آنجایی که یکی از نوآوری های اخیر استفاده از بازارهای مالی به منظور ریسک های فاجعه آمیز می باشد. از اینرو در این پایان نامه سعی شده است با استفاده از این نوآوری ها با طراحی اوراق اختیارات راهی نو جهت بیمه گردن نفت کش های ایرانی ارائه نمود. از آنجایی که بر...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied In Vitro Toxicology
سال: 2020
ISSN: 2332-1512,2332-1539
DOI: 10.1089/aivt.2020.29025.com